A real-time reverse-transcriptase PCR (RT-PCR) technique was developed for the rapid and specific detection and enumeration of viable Alternaria spp. in foodstuffs. The method uses Alernaria-specific primers and probe targeting the internal transcribed spacer regions ITS1 and ITS2 of the rRNA gene.

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Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is Results more widely used to detect HLA-DRb1*04 alleles. Addi- tionally, PCR-SSOP and Luminex methods are described The presence of a 259-bp specific band along with 434-bp as well for identifying the HLA alleles. Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is widely used to detect HLA-B * 27 [20].In this study, we developed an in-house PCR-SSP test which amplifies all the HLA-B * 27 alleles (27:01-27:73) except B * 27:18 and B * 27:23, which have not been reported from Asian population. 2020-05-23 · Solid-phase PCR (SP-PCR) is a unique PCR technique that allows amplification of target nucleic acids on a solid support where one or both primers are immobilized on the surface. The spatial separation of the primers minimizes significantly undesirable primer interactions, thereby preventing the formation of primer-dimers and allowing higher multiplexing amplification.

Pcr ssp technique

Different types of PCR The Polymerase Chain Reaction (PCR). © 2006 Sumanas, Inc. KEYWORDS: Polymerase chain reaction, DNA amplification, Taq polymerase, genomics 27 Jun 2016 Thus, a number of different techniques have been recently applied to the Discordant samples were confirmed by the PCR-SSP typing method 15 Apr 2015 However, differences in specificity were recorded, i.e. 84.3%, 97.2% and >99% for flow cytometry, qPCR and SSP PCR CE respectively. Our data 13 Oct 2006 antigen polymorphism by sequence-based and PCR-SSP methods in Using this method, we were able to characterize the entire Meishan av C Håkansson · 2017 — 20 olika DNA prover, Olerup SSP® HLA-B*57:01 och Inno-train HLA-READY House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients. We have developed a polymerase chain reaction-based technique, using sequence- specific primers (PCR-SSP) for HLA typing, starting with the HLA class II RNA is extracted from respiratory specimens, amplified using RT-PCR and detected using fluorescent generation sequencing or real-time RT-PCR methods [1,11]. Several assays that detect the Staphylococcus aureus ssp. aureus.

PCR-SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy-to-handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost.

The BAGene PCR sequence-specific priming (PCR-SSP) kits for weak D and partial D can be used to supplement serology in the investigation of weak or discrepant D findings. This method is based on the effect The techniques (PCR and TCBS‐1 plating) followed in this study are faster than the conventional API 20E and API CH50 used for the biochemical characterization of the P. damsela ssp. piscicida. The cost and labour involved in this method is less when compared with previous reports and commercially available kits and hence is suitable for use in all laboratories.

PCR-SSP technique utilizes oligonucleotide primers to start the PCR that have sequences complimentary to known sequences, which are characteristic to certain HLA specificities. For e.g. the primers

In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India. PMCID: PMC3880732 PMID: 24490069 [PubMed] In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India. Background.

ZERO BIAS - scores, article reviews, protocol conditions and more In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India. Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27.
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Application of a Simple In-House PCR-SSP Technique for HLA-B 27 Typing in Spondyloarthritis Patients DevrajJ.Parasannanavar, 1 AnjaliRajadhyaksha, 2 andKanjakshaGhosh 3 NationalInstituteofNutrition,ICMR,JamaiOsmania,Tarnaka,Hyderabad,AndhraPradesh,India Department of Medicine, King Edward Memorial Hospital Campus, Parel, Mumbai , India Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is Results more widely used to detect HLA-DRb1*04 alleles. Addi- tionally, PCR-SSOP and Luminex methods are described The presence of a 259-bp specific band along with 434-bp as well for identifying the HLA alleles. PCR techniques applied to Hematodinium spp.

What does PCR-SSP stand for? PCR-SSP abbreviation stands for Polymerase Chain Reaction with Sequence-Specific Primer.
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PCR SSP and PCR- SSO (principles) I'm doing a comparison of the two techniques, SSP (Sequence Specific Primers) and SSO (Sequence Specific Oligonucleotides), but I cannot seem to grasp the full principle in both (I understand the point of what we do and what we end up with, but I'm lacking the basic principle of what happens inbetween..).

2006;67(3):233-6. [ Links ] 47 Parasannanavar DJ, Rajadhyaksha A, Ghosh K. Application of a simple in-house PCR-SSP technique for HLA-B* 27 typing in spondyloarthritis patients. Arthritis.